Development and evaluation of microsatellite markers for a native prairie perennial, Echinacea angustifolia (Asteraceae)1
نویسندگان
چکیده
UNLABELLED PREMISE OF THE STUDY Microsatellite loci for the native prairie perennial Echinacea angustifolia were developed and evaluated for future use in population structure and paternity studies. • METHODS AND RESULTS A total of 50 trinucleotide microsatellite regions were identified though an enrichment protocol that prescreens for microsatellite repeats before ligating into a vector. Of these, 11 loci were polymorphic and in Hardy-Weinberg equilibrium in three populations with varying numbers of plants. The loci had between three and 14 alleles and collectively provided high paternity exclusion probabilities. • CONCLUSIONS These sets of microsatellite primers will provide researchers and land managers with valuable information on the population genetic structure and gene flow between fragmented prairie populations.
منابع مشابه
The Use of RAPDs for Assessment of Identity, Diversity, and Quality of Echinacea
The genus Echinacea, Asteraceae, is comprised of nine species, which are perennial herbs indigenous to North America and which have been traditionally used as medicinal plants for centuries. Three Echinacea species, E. angustifolia DC var. angustifolia, E. purpurea (L.) Moench, and E. pallida (Nutt.) Nutt., are currently being traded internationally in the natural products market. Echinacea pro...
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